Validation of the decomposed rice straw by using the above mentioned isolates was done in a greenhouse experiment. Seeds were placed in sterile autoclaved soils filled in 12 inch x 12 inch pots (each pot having 2.5 kg of autoclaved soil and 500g of decomposed rice straw by using respective Trichoderma strains) and incubated under ambient conditions (25°C). For control treatment, seeds were sown in the pots having equal amount of soil as in treated pots and 500g of decomposed rice straw without application of Trichoderma strain.
The germinability of the seeds in greenhouse was examined each day to calculate mean germination time till 8th days of sowing.
On 8th day, germination was determined as the ratio of the number of normal seedlings to the total number of seeds and expressed as percentage. On day eight of germination, ten normal seedlings were selected randomly in each treatment from all replications for measurement of shoot and root lengths. Seedlings used for recording seedling length from each replication were subsequently weighed after removing the cotyledons and the fresh weight was expressed in gram per 10 seedlings.
These seedlings were subsequently dried in an hot air oven at 55±1°C for 72 h and the dry weight was expressed in mg per 10 seedlings.
Chlorophyll content of the plants grown with decomposed rice straw by using respective Trichoderma strains in greenhouse was determined by following the procedure of Porra (2002).
Chopped fresh leaf tissue of 0.1 g was transferred to a capped measuring tube containing 25 ml of 80% acetone and kept inside a refrigerator at 40C for 48 h. Measurements were made using a spectrophotometer (Model- Evolution 300 UV-VIS; Double Beam Spectrophotometer, Thermo Fisher Scientific) at a wavelength of 663 nm and 645 nm to calculate chlorophyll a and chlorophyll b content, respectively.
In-vitro experiment was carried out to check the effect of decomposed rice straw by using respective Trichoderma for evaluation of growth promotion by taking two direct seeded rice varieties in Kharif season of 2018 (i.e.”Naveen”). Observations on health of plant (i.e. shoot length, root length, fresh shoot weight, dry shoot weight, fresh root weight, dry root weight) was recorded after 45 days and grain weight, yield/hill and yield/plot were recorded after harvesting.
Expression of stress related enzymes (i.e. catalase, peroxidase and superoxide dismutase) produced by the plants grown with decomposed rice straw by using respective Trichoderma strains were analyzed according to Nounjana et al., (2012).
Statistical analysis were performed by using the Statistical Analysis Software (SAS) of Indian Agricultural Statistics Research Institute (IASRI), New Delhi through the portal www.iasri.res.in/sscnars/-. Percentage of Seed germination data were ARCSINE transformed. The other seed quality parameters were analyzed without any transformation. All the data were subjected to one-way classified analysis of variance (ANOVA) and means of treatments were compared based on Tukey’s honestly significant difference test (HSD) at 0.05 probability level using SAS. All the experiments repeated at least three times.
Remember! This is just a sample.
You can get a custom paper by one of our expert writers.
Get your custom essay
Helping students since 2015
Essay Writing Service Features
Our Experience
No matter how complex your assignment is, we can find the right professional for your specific task. Contact Essay is an essay writing company that hires only the smartest minds to help you with your projects. Our expertise allows us to provide students with high-quality academic writing, editing & proofreading services.
Free Features
Free revision policy
$10Free bibliography & reference
$8Free title page
$8Free formatting
$8How Our Essay Writing Service Works
First, you will need to complete an order form. It's not difficult but, in case there is anything you find not to be clear, you may always call us so that we can guide you through it. On the order form, you will need to include some basic information concerning your order: subject, topic, number of pages, etc. We also encourage our clients to upload any relevant information or sources that will help.
Complete the order form
Once we have all the information and instructions that we need, we select the most suitable writer for your assignment. While everything seems to be clear, the writer, who has complete knowledge of the subject, may need clarification from you. It is at that point that you would receive a call or email from us.
Writer’s assignment
As soon as the writer has finished, it will be delivered both to the website and to your email address so that you will not miss it. If your deadline is close at hand, we will place a call to you to make sure that you receive the paper on time.
Completing the order and download