If the temperature is too high then the enzyme will denature. This means that the active site will be damaged, and will no longer fit with the substrate. This means that no reaction will occur.
It is unlikely, with a higher concentration of enzyme than substrate, that the substrate will be queuing, and this means that the reaction will happen faster. If the concentration of the enzyme is lower than that of the substrate then the reaction will be slower as the enzyme will be working at Vmax and the substrate will be queuing.
If it is reversible it means that if the substrate concentration is increased then there will be less inhibition, speeding the reaction up. If the inhibition is non-competitive irreversible then the inhibitor will bind to the enzyme’s active site and remain there, even if substrate concentration is increased. This will prevent a reaction from occurring.
Sometimes the inhibitor will bind to the enzyme but not with the active site. This is seen as non-competitive inhibition and while bonded no reaction can occur.
O2 Collected (cm3) Amount of H2O2 (ml) Amount of Water (ml) 1These results show that as the concentration of H2O2 increased, the amount of O2 collected increased. This means that my prediction is most likely to be correct although there is a chance that the results above are anomalous as the results were only taken once and not three times like they will be in the actual test.
A prediction is important as it provides a chance to see what will happen in the experiment and gives the opportunity for any errors, with the way the experiment is carried out or with the materials used, to be changed before the proper experiment is carried out.
In the boiling tube will be a piece of tubing that the O2 will pass through and then it will collect in the measuring cylinder. Every 10 seconds for 2 minutes the O2 collected will be recorded so that the rate of reaction can be worked out. To alter the substrate concentration, the H2O2 will be mixed with water. The first experiment will contain 1ml of H2O2 and 4ml of water, and then the second will have 2mls of H2O2 and 3ml of water. Using 5ml of solution means that it is easy to change concentration, decreasing one by a fifth and increasing the other by a fifth.
Throughout the experiment a water bath will keep the temperature at a constant of 37i?? C. This ensures that the experiment is fair as each one will be carried out at the same temperature, necessary because temperature affects the rate of an enzyme reaction. Each experiment will be carried out three times so that any anomalous results will be obvious. The data that is collected will be put into results tables and then a graph will be produced with Time in seconds on the x-axis and O2 collected on the y-axis. When using Hydrogen Peroxide it is important that many aspects of safety are observed.
H2O2 is corrosive and can cause burns in concentrations of 5.9M and irritation to skin and eyes in concentrations stronger or equal to 1. 5M. Therefore it is important to wear goggles to protect the eyes, and a lab coat to protect skin. If the H2O2 does get onto the skin or clothes then, if in large quantities, remove the clothing and wash the area of skin with lots of water. If H2O2 gets into the eyes then the eyes must be rinsed for 10-15 minutes and then medical attention should be sought. With any chemical it should not be swallowed. However, if it is swallowed then the mouth should be washed out and 2-3 glasses of water should be drunk.
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