Abstract:
In this experiment, I extracted DNA from the cells of Green Split Peas and Chicken Livers. I used different variables for each. First, I did the experiment with all materials being cold. The second time I did the experiment with materials at room temperature. My objective was to see which method would extract more DNA. The results were that the materials being colder extracted more DNA than the room temperature materials. In either case, I was able to extract more from the Green Split Peas than the Chicken Livers both times.
Introduction:
DNA is short for deoxyribonucleic acid. Nucleic acid, which is the genetic material determining the makeup of all living cells and many viruses. It consists of two long strands of nucleotides linked together in a structure resembling a ladder twisted into a spiral. The rungs of the ladder are made up of nucleotides: adenine (A), thymine (T), cytosine (C), and guanine (G). DNA can replicate itself. DNA also serves as a template for synthesis of RNA in the presence of RNA polymerase. (APA, Dictionary. com)
Get Help With Your Essay
If you need assistance with writing your essay, our professional essay writing service is here to help!
Essay Writing Service
DNA is located in the chromosomes in of the human body. It is like blueprints or instructions for hair color, eye color, height, pretty much everything. DNA can be used to identify criminals with unbelievable exactness when genetic evidence exists. Similarly, DNA can be used to clear suspects and clear persons wrongly accused or convicted of crimes. In all, DNA is ever-increasingly crucial to ensuring precision and fairness in the criminal justice system. (The United States Department of Justice) DNA is used in a few ways to solve crimes. Comparing a suspects DNA to DNA found at the scene, or it can be put in the database to try to find the offender’s match. DNA can also be used to identify victims.
Materials and Methods:
Chicken Livers
Green Split Peas
91% Rubbing Alcohol
Meat Tenderizer
Salt
Blender
4 small glass Pyrex bowels
Small strainer
Wooden Chopstick
Measuring cup
Liquid soap
Put in a blender: 1/2 cup of split peas (with the chicken livers I used 3 medium sized livers. ), 1/8 teaspoon salt, 1 cup cold water
Blend on high for 15 seconds
Pour through a strainer into measuring cup.
Add 2 tablespoons liquid soap and swirl to mix.
Let the mixture sit for 5-10 minutes.
Pour the mixture into glass bowls, each about 1/3 full.
Add a pinch of meat tenderizer and swirl very carefully
Tilt bowls and pour in rubbing alcohol. (Until it forms a layer on top of mixture)
Results:
The first experiment I used very cold materials, with the peas and with the chicken livers. After blending the peas, I got a very light green colored water solution. After straining, there was a lot of shell like material at the bottom of the blender. I then added the 2 tablespoons of liquid soap to the mixture and allowed it to sit for about 10 minutes. I then transferred the “soup” into the Pyrex bowls, where I added a pinch of meat tenderizer and stirred very gently with the wooden chopstick. After stirring the meat tenderizer in, I then added the rubbing alcohol until it formed a layer on top of the mixture. Almost immediately, I was able to see the white colored stringy material in the peas. These experiments also produced 3 layers: debris at the bottom, water in the middle, DNA floating at the top. I was able to transfer the DNA to another Pyrex bowl with alcohol in it to get a better look.
After blending the livers, I got a very reddish, beige colored water solution. After straining, there was liver material at the bottom of the blender. I then added the 2 tablespoons of liquid soap to the mixture and allowed it to sit for about 10 minutes. I then transferred the “soup” into the Pyrex bowls, where I added a pinch of meat tenderizer and stirred very gently with the wooden chopstick. After stirring the meat tenderizer in, I then added the rubbing alcohol until it formed a layer on top of the mixture. Almost immediately, I was able to see the white colored stringy material in the livers. These experiments also produced 3 layers: debris at the bottom, water in the middle, DNA floating at the top I was able to transfer the DNA to another Pyrex bowl with alcohol in it to get a better look.
The second experiment I used almost room temperature materials with the peas and with the chicken livers. After blending the peas, I got a very light green colored water solution. After straining, there was a lot of shell like material at the bottom of the blender. These experiments also produced 3 layers: debris at the bottom, water in the middle, DNA floating at the top. I then added the 2 tablespoons of liquid soap to the mixture and allowed it to sit for about 10 minutes. I then transferred the “soup” into the Pyrex bowls, where I added a pinch of meat tenderizer and stirred very gently with the wooden chopstick. After stirring the meat tenderizer in, I then added the rubbing alcohol until it formed a layer on top of the mixture. This time there was hardly any of the white stringy material I found in the first experiments, and barely anything to transfer.
After blending the livers, I again got a very reddish, beige colored water solution. After straining, there was a lot of material at the bottom of the blender. I then added the 2 tablespoons of liquid soap to the mixture and allowed it to sit for about 10 minutes. I then transferred the “soup” into the Pyrex bowls, where I added a pinch of meat tenderizer and stirred very gently with the wooden chopstick. After stirring the meat tenderizer in, I then added the rubbing alcohol until it formed a layer on top of the mixture. These experiments also produced 3 layers: debris at the bottom, water in the middle, DNA floating at the top. This time there was hardly any of the white stringy material I found in the first experiments, and yet again, barely anything to transfer.
Discussion:
My results were surprising. These experiments also produced 3 layers: debris at the bottom, water in the middle, DNA floating at the top.
It really made a difference in the outcome just by using colder materials. Using ice-cold water and ice-cold alcohol will increase the yield of DNA. “Low temperatures protect the DNA by slowing down the activity of enzymes that could break it apart. The cold alcohol helps the DNA precipitate (solidify and appear) more quickly. Why would a cell contain enzymes that destroy DNA? These enzymes are present in the cell cytoplasm (not the nucleus) to destroy the DNA of viruses that may enter our cells and make us sick. A cell’s DNA is usually protected from such enzymes (called DNases) by the nuclear membrane, but adding detergent destroys that membrane. ” (gslc. genetics. utah. edu)
Out of the two, the warmer water experiments eliminated the most debris, but were not very successful at extracting as much DNA material.
References:
American Psychological Association: dna. (n. d. ). Dictionary. com Unabridged. Retrieved April 12, 2015, from Dictionary. com website:http://dictionary. reference. com/browse/dna
Explorable : https://explorable. com/who-discovered-dna
Learn. Genetics, The Genetic Science Learning Center, Retrieved from: http://learn. genetics. utah. edu/content/labs/extraction/howto/faq/
Essay Writing Service Features
Our Experience
No matter how complex your assignment is, we can find the right professional for your specific task. Contact Essay is an essay writing company that hires only the smartest minds to help you with your projects. Our expertise allows us to provide students with high-quality academic writing, editing & proofreading services.
Free Features
Free revision policy
$10Free bibliography & reference
$8Free title page
$8Free formatting
$8How Our Essay Writing Service Works
First, you will need to complete an order form. It's not difficult but, in case there is anything you find not to be clear, you may always call us so that we can guide you through it. On the order form, you will need to include some basic information concerning your order: subject, topic, number of pages, etc. We also encourage our clients to upload any relevant information or sources that will help.
Complete the order form
Once we have all the information and instructions that we need, we select the most suitable writer for your assignment. While everything seems to be clear, the writer, who has complete knowledge of the subject, may need clarification from you. It is at that point that you would receive a call or email from us.
Writer’s assignment
As soon as the writer has finished, it will be delivered both to the website and to your email address so that you will not miss it. If your deadline is close at hand, we will place a call to you to make sure that you receive the paper on time.
Completing the order and download